PCR Equipment and Supplies
As One International Inc Taq DNA Polymerase 1.1x Master Mix, 1.5 mM MgCl2 (final concentration), 20,000Rxn
AS ONE's Taq DNA Polymerase Pre-Mix is a ready-to-use 1.1X reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq DNA Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are present in Taq DNA Polymerase Mix. Each reaction requires 45 uL of the 1.1X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL.Taq DNA Polymerase Pre-Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Biotium pS2 / pNR-2 / TFF1 (Estrogen-Regulated Protein)(rTFF1/1091), CF568 conjugate, 0.1mg/mL
Recognizes a polypeptide of 6. 5 kDa, identified as pS2 estrogen-regulated protein. Its epitope is located in the c-terminus of human pS2 protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelih
As One International Inc Taq DNA Polymerase 2x Master Mix, 1.5 mM MgCl2 (final concentration), 10,000Rxn
AS ONE's Taq DNA Polymerase Pre-Mix is a ready-to-use 2.0X reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq DNA Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are present in Taq DNA Polymerase Mix. Each reaction requires 25 uL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL. Taq DNA Polymerase Pre-Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Meridian Life Science Gly-Free DNA Helicase -HC, 200UL
Glycerol-Free DNA Helicase (HC) Kit is a multi-domain enzyme with both ATPase and helicase activities. It unwinds double-stranded DNA with 3’ to 5’ with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Helicase works in coordination with an accessory protein, methyl-directed mismatch repair (MutL) protein that stimulates the helicase activity. Glycerol-Free DNA Helicase (HC) has been optimized to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilization. This gives you the flexibility to confidently store mixtures at ambient temperature or produce diagnostic assays that rely on miniaturized reaction components, such as point-of-care assays.
Biotium CD43 (T-Cell Marker) (rSPN/1094), Biotin conjugate, 0.1mg/mL
This antibody recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low ab
Meridian Life Science Glycerol-Free Taq HS High, 5ML
Supplied as a blend of high‑performance glycerol‑free Taq DNA Polymerase with a Taq hot-start antibody and is suitable for difficult, multiplex PCR that require fast amplification, whilst maintaining both high specificity and sensitivity.Glycerol-Free Taq HS High Conc. is ideal for PCR kit manufactures and PCR molecular test facilities, requiring large amounts of DNA polymerases. The highly concentrated Glycerol-Free Taq HS High Conc. allows for concentrated bulk production of PCR master mixes and the glycerol free format is useful when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer and 10x Combination Buffer both Magnesium free, 500U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes magnesium free 10x Ammonium Buffer and 10x Combination Buffer.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer and 10x Standard Buffer both Magnesium and detergent free, 1,000U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes Tween/Triton/magnesium free 10x Ammonium Buffer and 10x Standard Buffer.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer and 10x Standard Buffer, 5,000U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes 10x Ammonium Buffer and 10x Standard Buffer.
Meridian Life Science Taq HS DNA Polymerase, 100UL
Taq HS DNA Polymerase is an antibody-mediated hot-start DNA polymerase provided with an optimized buffer system containing dNTP, MgCl2, stabilizers, and PCR enhancers at optimal concentrations which eliminates the need for optimization. The DNA polymerase has been developed to give more robust amplification than other polymerases allowing it to perform well with challenging templates in the presence of PCR inhibitors, making it ideal for fast PCR reactions across a range of templates, without compromising specificity or yield.
Meridian Life Science dNTP Set 100mM in Lithium salt, 1ML
Ready-to-use Ultra pure sodium salt dNTPs are available in multiple concentrations and supplied in purified water at pH 7.5. dNTPs are enzymatically synthesized from quality raw materials, using highly specific production systems. The manufacturing process eliminates impurities and PCR-specific inhibitors such as modified nucleotides, tetraphosphates, and pyrophosphates commonly observed in other commercially available dNTP products (>99% purity determined by quantitative HPLC) and are perfectly suited for even the most demanding applications, such as Long PCR or highly sensitive qPCR.
As One International Inc Taq DNA Polymerase 1.1x Master Mix, 1.5 mM MgCl2 (final concentration), 500Rxn
AS ONE's Taq DNA Polymerase Pre-Mix is a ready-to-use 1.1X reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq DNA Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are present in Taq DNA Polymerase Mix. Each reaction requires 45 uL of the 1.1X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL.Taq DNA Polymerase Pre-Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
As One International Inc Taq DNA Polymerase 5 U/ul, 10x Ammonium Buffer and 10x Standard Buffer, 2,500U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Included with Taq are 10x Ammonium Buffer and 10x Standard Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul, 10x Ammonium Buffer and 10x Combination buffer both Magnesium and detergent free , 2,500U
AS ONE's Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Taq Polymerase includes Tween/Triton/Magnesium free 10x Ammonium Buffer and 10x Combination Buffer.